Optimum Culture Conditions for Production byPseudomonas chlororaphisB23 of Nitrile Hydratase
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چکیده
منابع مشابه
Post-translational modifications in nitrile hydratase family
Fe-type nitrile hydratase (NHase) from Rhodococcus sp. N771 has a novel non-heme iron active center with two post-translationally modified cysteine residues, cysteine-sulfenic and -sulfinic acids. The modified residues are involved in the sequence motif, Cys-Xxx-Leu-Cys-Ser-Cys (Xxx = Ser(Fetype)/Thr(Co-type)), which is conserved among all known NHases as well as in the homologous enzyme, thioc...
متن کاملAldoxime dehydratase co-existing with nitrile hydratase and amidase in the iron-type nitrile hydratase-producer Rhodococcus sp. N-771.
We identified an aldoxime dehydratase (Oxd) gene in the 5'-flanking region of the nitrile hydratase-amidase gene cluster in the photoreactive iron-type nitrile hydratase-producer, Rhodococcus sp. N-771. The enzyme showed 96.3%, 77.6%, and 30.4% identities with the Oxds of Rhodococcus globerulus A-4, Pseudomonas chlororaphis B23, and Bacillus sp. OxB-1, respectively. The enzyme was expressed in ...
متن کاملMolecular dynamics simulations of the photoactive protein nitrile hydratase.
Nitrile hydratase (NHase) is an enzyme used in the industrial biotechnological production of acrylamide. The active site, which contains nonheme iron or noncorrin cobalt, is buried in the protein core at the interface of two domains, alpha and beta. Hydrogen bonds between betaArg-56 and alphaCys-114 sulfenic acid (alphaCEA114) are important to maintain the enzymatic activity. The enzyme may be ...
متن کاملfaciliting lexical access for the fluent production of speech
the hypothesis is that recent and frequent exposure to lexical items leads to a more fluent production of speech in terms of rate of speech. to test the hypothesis,a one-way anova experimental design was carried out. 24 sednior students of efl participated in a one-way interview test. data analyses revealed that those who were exposed frequently to the lexical items over a week prior to inter...
15 صفحه اولfacilitating lexical access for the fluent production of speech
the hypothesis is that recent and frequent exposure to lexical items leads to a more fluent production of speech in terms of rate of speech. to test the hypothesis , a one- way anova experimental design was carried out. 24 senior student of efl participated in a one-way interview test. data analyses revealed that those who were exposed frequently to the lexical items over a week prior to inte...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1986
ISSN: 0002-1369
DOI: 10.1080/00021369.1986.10867844